Overexpression of CfPIP1-1,CfPIP1-2,and CfPIP1-4 genes of Catalpa fargesii in transgenic Arabidopsis thaliana under drought stress

• 作者: Wenjun Ma Yao Xiao Yuan Li Pan Hu Zhi Wang Guijuan Yang Junhui Wang
• 来源期刊: 林业研究（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: ORIGINAL PAPERS
• 页码范围: 285-296
• 页数: 12页
• 语种: 英文

摘要

Catalpa fargesii is an important economic tree species used for furniture and timber production because of its high density and hardness.Its survival and growth are severely affected and primarily limited by drought stress.Thus,to better understand the mechanism of drought resist-ance in C.fargesii,we used qRT-PCR to reveal significantly different expression of three plasma membrane intrinsic protein genes:CfPIP1-1,CfPIP1-2 and CfPIP1-4.We then cloned their full-length cDNA sequences and character-ized the encoded proteins.We analyzed the genes phylo-genetically and predicted conserved motifs,domains,and secondary and tertiary structures.To verify the function of the CfPIP1 genes further,we ectopically expressed CfPIP1 transgenes in Arabidopsis thaliana.The results showed that CfPIP1-1,CfPIP1-2 and CfPIP1-4 had several characteris-tics of aquaporins.The transgenic plants grew better than the WT plants did under drought stress,and overexpres-sion of the CfPIP1 genes increased the plant water content and resistance to drought.Thus,CfPIP1-1,CfPIP1-2 and CfPIP1-4 of C.fargesii play key roles in regulating the intra-cellular and extracellular water balance and in mediating the plant response to drought.