Effects of chimeric intron on the epithelial-mesenchymal transformation of non-small cell lung cancer PC-9

• 作者: Liang Liao Guo-Hui Yang
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Original article
• 页码范围: 1-5
• 页数: 5页
• 语种: 英文

摘要

Objective:To investigate the effects of Intron on the EMT capability of non-small cell lung cancer cell line PC-9. Methods:Firstly, using the psiCHECK-2 plasmid as a basic framework to construct the recombinant plasmid of psiCHECK-2-Intron dual-luciferase reporter gene;secondly, the psiCHECK-2-Intron and psiCHECK-2 were transfected into PC-9 cells respectively. The migration and invasion abilities of PC-9 cells were analyzed by Matrigel assay. The expression changes of EMT related hallmarks, including N-cadherin, β-catenin and snail, were detected by qRT-PCR and Western Blotting. Results:Compared with the control group, the migration and invasion abilities of PC-9 cells in Intron group significantly decreased(p<0.001). The expression of N-cadherin, β-catenin and snail also down-regulated (p<0.001). Conclusion:The introns could inhibit the EMT of PC-9 cells.