Integrated System for Combined Optical Coherence Tomography-Raman Spectroscopy of Neocaridina denticulate sinensis

• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Marine and Atmospheric Sciences
• 页码范围: 94-100
• 页数: 7页
• 语种: 英文

摘要

Optical coherence tomography (OCT) and Raman spectroscopy (RS) can be complementary biological tissue optical analysis methods.To study the internal structure and tissue compositions of biological samples,an OCT-RS system was built to carry out OCT section imaging and RS analysis in common.Neocaridina denticulate sinensis were collected regularly for morphological observation by OCT imaging and biochemical investigation based on the Raman spectra.The internal structure of the N.denticulate sinensis was imaged by OCT,and the morphology of the tissues and the position in the body were distinguished according to the gray scale changes.The imaging depth along the vertical direction of Z-axis in N.denticulate sinensis is about 1.60mm.RS detection was selectively performed based on the OCT images.The main Raman peaks of the rostrum,the cephalothorax,the abdominal segment,and the telson section are at 1006,1156,1447,1491 and 1515 cm-1,which are identified as proteins and amino acids.The presence of 1497 cm-1 at the cephalothorax is different from other parts,probably due to the presence of organs such as ovary,whose compositions are different from those of other tissues.The combination of optical coherence tomography and Raman spectroscopy can provide information about morphological and biochemical features of tissues,and has potential applications in biomedical detection and imaging.