Cytotoxicity of oleanane type triterpene from leaf extract of Pterospermum acerifolium(in vitro)and theoretical investigation of inhibitory signaling pathway

• 作者: Mohd Rehan Shafiullah Sultanat
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Original Articles
• 页码范围: 124-130
• 页数: 7页
• 语种: 英文

摘要

Objective:To evaluate the cytotoxic activity of taraxerol isolated from the leaves of Pterospermum aceri-folium and its EtOH extract against human breast,colon,and lung cancer cell lines and docking studies.Methods:The structures of the isolated compounds were elucidated by several spectroscopic methods such as 1H-NMR,13C-NMR,DEPT-135,COSY,HSQC,and HMBC.The extract and isolated compound were analyzed for cytotoxic activity on MDA-MB-231,BT-549,A-549,and SW-480 cancer cell lines by MTT assay.Molecular docking was performed on software such as Chem3D pro 12.0.2.1076,Discovery Studio Visualizer,Auto Dock Tools-1.5.6 and Auto dock vina.Results:The extract and isolated compound taraxerol both displayed excellent inhibitory activity(IC50:80 μg/mL for extract and 160 μg/mL for compound)on breast cancer cell(MDA-MB-231).The docking studies show a strong affinity with P13K(-9.8 Kcal/mol)and mTOR(-10.0 Kcal/mol).Conclusion:The results confirm that the extract and compound exhibited strong cytotoxicity on the MDA-MB-231 cancer cell.So,the extract and the compound may be useful in human chemotherapies.