Identification of a male-specific region(MSR)in Spinacia oleracea

• 作者: Hongbing She Zhaosheng Xu Helong Zhang Guoliang Li Jian Wu Xiaowu Waang Ying Li Zhiyuan Liu Wei Qian
• 来源期刊: 园艺学报（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Research Papers
• 页码范围: 341-346
• 页数: 6页
• 语种: 英文

摘要

Dioecy,the presence of male and female individual,has evolved independently from hermaphroditism in multiple flowering plant lineages.Spinach,an important leafy vegetable crop worldwide,is a dioecious species with an XY sex-determination system.Although some markers that are fully linked to male-determination locus were identified,the male-specific region(MSR)remained unclear.In this research,five male individuals and five female lines were resequenced to identify the male-specific region.We identified a region(～21kb)on chromosome 4(putative sex chromosome)where the five females had a low reads coverage,while the five males had high coverage.A KASP marker,SponR,developed from a single nucleotide polymorphism(SNP)closely linked to the MSR was co-segregating with the sex determination gene in the population of 958 individuals,suggesting that the MSR might be specifically present in male spinach plants.