Anaphylaxis effect and substance basis of honeysuckle extract

• 作者: Yu-fei Feng Guo-zhao Qin Zhong-xu Jing Yan-hong Wang Yan-yan Zhou
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Original Articles
• 页码范围: 403-409
• 页数: 7页
• 语种: 英文

摘要

Objective:To explore the anaphylaxis effect and anaphylaxis substances of honeysuckle.Methods:Rat peritoneal mast cells(PMC)were separated and purified,the cells were incubated with compound 48/80(0.02 g/L),physiological saline and honeysuckle extract(120 g/L)at 37℃for 0,15,30,45 and 60 min.Degranulation were observed by optical microscope and transmission electron micro-scope.Annexin V positive cell rate was detected by flow cytometry to reflect the degranulation rate of PMC.SD rats were supplied with honeysuckle extract by intravenous injection at a dose of 2.25 g/L.After administration,different parameters were analyzed,including the symptoms,histamine(HIS)and tryptase(MCT)levels,which were determined to explore the effect of anaphylaxis.Regression anal-ysis was used to calculate the relationships between the peaks and the pharmacological effects to explore potentially anaphylactoid components.Results:The percentage of Annxin V positive cells and the degranulation ratio were markedly elevated in PMC treated with honeysuckle extract for more than 15 min(P＜0.05).HIS and MCT level were signifi-cantly elevated after injection of honeysuckle extract for more than 15 min.Morphology of PMC and sys-temic symptoms were also changed compared with the controlled group(P＜0.05).Regression analysis was used to calculate the relationship between peaks and pharmacological effects,and to determine peaks 7,10 and 13 as possible anaphylactoid ingredients.Conclusion:This study established a prospective method to clarify the anaphylactoid components of honeysuckle extract,which would provide guidance for screening anaphylactoid components in tradi-tional Chinese medicine injections containing honeysuckle in the prescription.