Xiaokeping-containing serum suppresses high-glucose-induced proliferation of mesangial cells involved in the regulation of cell cycle progression and the p38 mitogen-activated protein kinase pathway

• 来源期刊: 中医杂志（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Research Articles
• 页码范围: 44-50
• 页数: 7页
• 语种: 英文
• DOI: 10.19852/j.cnki.jtcm.2021.01.006

摘要

OBJECTIVE:To investigate the efficacy of Xia-okeping(XKP)-containing serum on the prolifera-tion of high-glucose-induced mesangial cells(MCs)and the potential underlying mechanism.METHODS:XKP-containing serum was prepared by the intragastric administration of XKP in rats.HBZY-1 cells were cultured with normal glucose(NC group),high glucose(HG group),and high glu-cose with different XKP concentrations.Cell prolifer-ation was assessed by the 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide assay,and the cell cycle distribution was detected by flow cytometry.The expression of p38 mitogen-activat-ed protein kinase(p38MAPK)pathway compo-nents in MCs was detected by Western blotting and quantitative real-time polymerase chain reaction.RESULTS:The MC proliferation level in the high-glucose group was significantly higher than that in the normal control group,and XKP sup-pressed the HG-induced proliferation of MCs dose dependently.Moreover,flow cytometry revealed that XKP blocked cell cycle progression by inducing cell cycle arrest in G1 phase and inhibiting S phase entry.XKP down-regulated the protein and mRNA expression of p38MAPK in MCs(P＜0.05 vs HG).CONCLUSION:The present study demonstrated that XKP-containing serum inhibits high-glucose-induced proliferation of MCs by causing cell cycle arrest at G1 phase and inhibiting S phase entry.The underlying mechanism involves the down-regula-tion of the p38MAPK signaling pathway,providing a theoretical basis for the use of XKP to treat diabet-ic kidney disease.