Structure of plant photosystem I?light harvesting complex I supercomplex at 2.4 ? resolution

• 作者: Jie Wang Long-Jiang Yu Wenda Wang Qiujing Yan Tingyun Kuang Xiaochun Qin Jian-Ren Shen
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（7）
• 所属期刊栏目: Photosynthesis and Crop Physiology
• 页码范围: 1367-1381
• 页数: 15页
• 语种: 英文

摘要

Photosystem I (PSI) is one of the two photo-systems in photosynthesis, and performs a series of electron transfer reactions leading to the re-duction of ferredoxin. In higher plants, PSI is sur-rounded by four light-harvesting complex I (LHCI) subunits, which harvest and transfer energy effi-ciently to the PSI core. The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6 ? resolution, providing much information on the arrangement of proteins and cofactors in this complicated supercomplex. Here we have opti-mized crystallization conditions, and analyzed the crystal structure of PSI-LHCI at 2.4 ? resolution. Our structure showed some shift of the LHCI, es-pecially the Lhca4 subunit, away from the PSI core, suggesting the indirect connection and in-efficiency of energy transfer from this Lhca sub-unit to the PSI core. We identified five new lipids in the structure, most of them are located in the gap region between the Lhca subunits and the PSI core. These lipid molecules may play important roles in binding of the Lhca subunits to the core, as well as in the assembly of the supercomplex. The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting, transfer and assembly of this supercomplex.