Influences of ischemic postconditioning at different positions on oxidative stress of myocardial ischemia-reperfusion injury in rats

• 作者: Chao Wang Jian An Xiao-Hong Li Ye Du
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（6）
• 所属期刊栏目: Original article
• 页码范围: 1-4
• 页数: 4页
• 语种: 英文

摘要

Objective: To analyze the influences of locial ischemic postconditioning and remote limb ischemic postconditioning on oxidative stress response with myocardial ischemia reperfusion in rats. Methods: Thirty-two SD rats were randomly divided into Sham group, ischemia/ reperfusion (I/R) group, local ischemic postconditioning (LIPC) group, and remote limb ischemic postconditioning(RIPC) group, after 3 hourse reperfusion,the contents of serum creatinine kinase,MB isoenzyme(CK-MB), xanthine oxidase (XOD), superoxide dismutase (SOD), myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α) were measured. The 2,3,5-triphenyltetrazolium chloride(TTC) staining was carried out to evaluate the area of myocardial infarction,cardiac function was evaluated by echocardiography, and HE staining was performed to observe the morphology of myocardial cells. Results: Compared with the Sham group, the SOD contents of the I/R group, LIPC group, RIPC group reduced significantly (P <0.05),the XOD,MPO,TNF-α contents increased significantly (P <0.05); Compared with the I/R group, the TNF-α contents of the LIPC group reduced significantly (P <0.05), other oxidative stress indicators of the LIPC group had no significant differences; Compared with the I/R group, the MPO and TNF-α contents reduced (P <0.05), the SOD and XOD contents of the RIPC group had no significant differences;Compared with the LIPC group, the MPO contents reduced (P <0.05) in the RIPC group, other oxidative stress indicators had no significant differences. Compared with the Sham group, myocardial infarction area, CK-MB contents, LVIDs increased with the reduction of EF in I/R group, LIPC group, RIPC group (P <0.05), HE staining had differences; Compared with the I/R group,myocardial infarction area, CK-MB contents, LVIDd, LVIDs, EF and HE staining results had no significant differences in the LIPC group and the RIPC group; Compared with the RIPC group, the LIPC group had no significant differences. Conclusion: Remote limb ischemic postconditioning and local ischemic postconditioning can partially reduce the oxidative stress response,but does not significantly reduce myocardial infarction area, improve cardiac function.