The piRNA response to BmNPV infection in the silkworm fat body and midgut

• 作者: Min Feng Anna Kolliopoulou Yao-Hong Zhou Shi-Gang Fei Jun-Ming Xia Luc Swevers Jing-Chen Sun
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Original articles
• 页码范围: 662-679
• 页数: 18页
• 语种: 英文
• DOI: 10.1111/1744-7917.12796

摘要

Bombyx mori nucleopolyhedrovirus(BmNPV)is a DNA virus that causes huge losses to the silkworm industry but the piRNA responses during BmNPV infection in the silkworm remain uninvestigated.Here,silkworm piRNA profiles of uninfected and BmNPV-infected fat body and midgut were determined by high-through sequencing in the early stages of BmNPV infection.A total of2675 and 3396 genome-derived piRNAs were identified from fat body and midgut,respectively.These genome-derived piRNAs mainly originated from unannotated instead of transposon regions in the silkworm genome.In total,572 piRNAs were associated with 280 putative target genes in fat body and 805 piR-NAs with 380 target genes in midgut.Compared to uninfected tissues,322 and 129 piR-NAs were significantly upregulated in BmNPV-infected fat body and midgut,respectively.In addition,276 and 117 piRNAs were significantly downregulated.Moreover,differen-tially expressed(DE)piRNAs during BmNPV infection differed significantly between fat body and midgut.Putative DE piRNA-targeted genes were associated with"response to stimulus"and"environmental information processing"in fat body after infection with BmNPV,which may indicate an active piRNA response to BmNPV infection in fat body.This study may lay the foundation for future research of the potential roles of the piRNA pathway and specific piRNAs in BmNPV pathogenesis.