Nicotiana benthamiana LRR-RLP NbEIX2 mediates the perception of an EIX-like protein from Verticillium dahliae

• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: Plant-biotic Interactions
• 页码范围: 949-960
• 页数: 12页
• 语种: 英文

摘要

Verticillium wilt diseases caused by the soil-borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually.Here,we identified a novel ethylene-inducing xylanase (EIX)-like protein,VdEIX3,from V.dahliae,which exhibits immunity-inducing activity in Nicotiana benthamiana.In vitro-purified VdEIX3 can induce strong oxidative burst,activate the expression of defense-related genes,and increase resistance against oomycete and fungal pathogens in N.benthamiana.VdEIX3 orthologs of other Verticillium pathogens also induce cell death in N.benthamiana,which form a new type of EIX protein family that is distinct from the known EIX proteins.A leucine-rich repeat receptor-like protein,NbEIX2,regulates the perception of VdEIX3 in N.benthamiana.Our results demonstrate that VdEIX3 is a novel EIX-like protein that can be recognized by N.benthamiana NbEIX2,and also suggest that NbEIX2 is a promising receptor-like protein that is potentially applicable to transgenic breeding for improving resistance to Verticillium wilt diseases.