Sequencing-Based High-Throughput Neuroanatomy:From Map-seq to Bricseq and Beyond

• 作者: Xiaoyang Wu Qi Zhang Ling Gong Miao He
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: Insights
• 页码范围: 746-750
• 页数: 5页
• 语种: 英文

摘要

Dissecting inter-regional connectivity at the cellular level is a key step towards understanding the functional oper-ation scheme of a complex brain network.Large numbers of highly heterogeneous neurons are often packed within a small region.Traditional long-range projection tracing techniques mostly rely on the injection of viral or chemical tracers and the direct visualization of axon terminals at distant sites by optical imaging[1].The diverse projection patterns of individual neurons are often difficult to discern if many neurons in the same region are labeled in bulk with the same fluorescent or enzymatic label(Fig.1A).