Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies

• 作者: Peizhe Song Junbo Yang Chunling Wang Qiang Lu Linqing Shi Subiding Tayier Guifang Jia
• 来源期刊: 分子植物（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Research Articles
• 页码范围: 571-587
• 页数: 17页
• 语种: 英文

摘要

The biological functions of the epitranscriptomic modification N6-methyladenosine (m6A) in plants are not fully understood.CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m6A-binding YTH domain.Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m6A-binding function in alternative polyadenylation.Here,we characterized CPSF30-L as an Arabidopsis m6A reader whose m6A-binding function is required for the floral transition and abscisic acid (ABA) response.We found that the m6A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies,where CPSF30-L mainly recognizes m6A-modified far-upstream elements to control polyadenylation site choice.Deficiency of CPSF30-L lengthens the 3'untranslated region of three phenotypes-related transcripts,thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity.Collectively,this study uncovers a new molecular mechanism for m6A-driven phase separation and polyadenylation in plants.