Effect of Zinc Oxide Nanoparticles on Denitrification and Denitrifying Bacteria Communities in Typical Estuarine Sediments

• 作者: CHEN Xi XIANG Zhuangzhuang REN Zhaomeng HUANG Xiao LI Hui SUN Pengfei BAI Jie
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Marine and Atmospheric Sciences
• 页码范围: 599-607
• 页数: 9页
• 语种: 英文

摘要

For revealing the effects of increasing of zinc oxide nanoparticles(ZnO NPs)on denitrification and denitrifying bacteria communities in estuarine sediments,the surface sediments of two typical estuaries(the Yangtze River Estuary and the Yellow River Estuary)were added with medium concentration(170mgL-1)and high concentration(1700mgL-1)of ZnO NPs for anaerobic cul-ture in laboratory.The concentration of NO3-and NO2-,the reductase activity and denitrification rate were measured by physico-chemical analysis,nirS gene abundance and denitrifying bacteria communities by molecular biological methods.The results showed that ZnO NPs inhibited NO3-,NO2-reduction process and NO3-,NO2-reductase activity,and a stronger inhibition effect resulting from the higher ZnO NPs concentration.ZnO NPs decreased nirS gene abundance and community diversity of denitrifying bacteria.In addition,the inhibition degree of ZnO NPs on the denitrification process of sediments in different estuaries was different.These results were of great significance for evaluating the potential ecological toxicity and risks of nanomaterials in estuaries.