Inhibition of trehalase affects the trehalose and chitin metabolism pathways in Diaphorina citri(Hemiptera:Psyllidae)

• 作者: Hai-Zhong Yu Yu-Ling Huang Zhan-Jun Lu Qin Zhang Hua-Nan Su Yi-Ming Du Long Yi Ba-Lian Zhong Ci-Xiang Chen
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Original articles
• 页码范围: 718-734
• 页数: 17页
• 语种: 英文
• DOI: 10.1111/1744-7917.12819

摘要

The Asian citrus psyllid,Diaphorina citri is the principal vector of huang-longbing,which transmits Candidatus Liberibacter asiaticus.Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and develop-ment.The specific functions of this enzyme in D.citri have not been determined.In this study,three trehalase genes(DcTre1-1,DcTre1-2,and DcTre2)were identified based on the D.citri genome database.Bioinformatic analysis showed that DcTrel-1 and DcTrel-2 are related to soluble trehalase,whereas DcTre2 is associated with membrane-bound trehalase.Spatiotemporal expression analysis indicated that DcTrel-1 and DcTrel-2 had the highest expression levels in the head and wing,respectively,and DcTre2 had high ex-pression levels in the fat body.Furthermore,DcTre1-1 and DcTre1-2 expression levels were induced by 20-hydroxyecdysone and juvenile hormone Ⅲ,but DcTre2 was unaf-fected.The expression levels of DcTre1-1,DcTre1-2,and DcTre2 were significantly up-regulated,which resulted in high mortality after treatment with validamycin.Trehalase activities and glucose contents were downregulated,but the trehalose content increased after treatment with validamycin.In addition,the expression levels of chitin metabolism-related genes significantly decreased at 24 and 48 h after treatment with validamycin.Furthermore,silencing of DcTre1-1,DcTre1-2,and DcTre2 reduced the expression levels of chitin metabolism-related genes and led to a malformed phenotype of D.citri.These re-sults indicate that D.citri trehalase plays an essential role in regulating chitin metabolism and provides a new target for control of D.citri.