Antimicrobial peptide hepcidin contributes to host defense of Centropristis striata against Vibrio harveyi challenge

• 作者: Xiaowan Ma Ying Qiao Yanxiang Shao Chao Chen
• 来源期刊: 海洋学报（英文版）
• 年，卷（期）: 2021,（6）
• 所属期刊栏目: Marine Biology
• 页码范围: 61-66
• 页数: 6页
• 语种: 英文

摘要

Hepcidins are small cysteine-rich antimicrobial peptides that play a vital role in immunity against pathogeninvasion. Here, a hepcidin (Cshep) from Centropristis striata was described, which is considered as a valuable aquaculture marine species in China. The open reading frame consisted of 273 bp. Eight conserved cysteine residues were identified. Phylogenetic analysis showed that Cshep had a relatively close relationship with the hepcidin from Epinephelus moara. Quantitative real-time PCR analysis demonstrated that Cshep was highly expressed in liver and significantly up-regulated when challenged with Vibrio harveyi. In addition, the synthetic Cshep peptide had a high antimicrobial activity against V. harveyi, but low against other pathogenic bacteria tested in this study. The killing kinetics analysis revealed that Cshep had a fast bactericidal effect on V. harveyi. These results suggested that Cshep may be involved in the immune response of C. striata against V. harveyi infection.