Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity

• 作者: Xiangqi Fan Haiyan Hu Daiwen Chen Bing Yu Jun He Jie Yu Junqiu Luo Erik Eckhardt Yuheng Luo Jianping Wang Hui Yan Xiangbing Mao
• 来源期刊: 畜牧与生物技术杂志（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: ANIMAL NUTRITION AND FEEDSTUFFS
• 页码范围: 1125-1137
• 页数: 13页
• 语种: 英文

摘要

Background:Lentinan (LNT) may regulate many important physiological functions of human and animals. This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs. Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n=14). RV infection was executed on d 15. After extracting polysaccharides from LNT product, its major monosaccharides were analyzed. Then, LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells. Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P<0.05). The polysaccharide was isolated from LNT product, which molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P<0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P<0.05). Conclusion:Those results suggest that LNT administration increases the piglets' resistance to RV-induced stress, likely by supporting intestinal immunity.