Compact long-working-distance laser-diode-based photoacoustic microscopy with a reflective objective

• 作者: Lijun Deng Qi Chen Yang Bai Guodong Liu Lüming Zeng Xuanrong Ji
• 来源期刊: 中国光学快报（英文版）
• 年，卷（期）: 2021,（7）
• 所属期刊栏目: Biophotonics
• 页码范围: 56-61
• 页数: 6页
• 语种: 英文
• DOI: 10.3788/COL202119.071701

摘要

Photoacoustic microscopy[PAM]has quickly developed into a noninvasive biomedical imaging technique to achieve detec-tion,diagnosis,and monitoring.Compared with Q-switched neodymium-doped yttrium aluminum garnet or optical para-metric oscillator lasers,a low-cost and small-size laser diode[LD]used as an alternative light source is conducive to achieving the miniaturization and integration for preclinical transformation.However,the LD's low peak output power needs the high numerical aperture objective to attain tight focus,which limits the working distance[WD]of the system in only 2-3 mm,resulting in not achieving the backward coaxial confocal mode.Here,we present a compact visible LD-based PAM system with a reflective objective to achieve a 22 mm long WD and a 10 μm lateral resolution.Different depth subcutaneous microvascular networks in label-free mouse ears have successfully reappeared in vivo with a signal-to-noise ratio up to 14 dB by a confocal alignment.It will be a promising tool to develop into a handy tool for subcutaneous blood vessel imaging.