Implications from γ-globulin adsorption onto cation exchangers fabricated by sequential alginate grafting and sulfonation

• 来源期刊: 中国化学工程学报（英文版）
• 年，卷（期）: 2021,（1）
• 所属期刊栏目: Separation Science and Engineering
• 页码范围: 121-125
• 页数: 5页
• 语种: 英文

摘要

Our previous work proved that high adsorption capacity and uptake rate of lysozyme were achieved on alginate (Alg)-grafted resin with an ionic capacity (IC) of 240 mmol·L-1 (Alg-FF-240).Moreover,the salt-tolerant feature of Alg-FF-230 was improved by using sequential alginate grafting and sulfonation strategy.Inspired by the enhanced adsorption performance of lysozyme,we have herein proposed to investigate the static and dynamic adsorption behaviors of γ-globulin on a series of Alg-grafted resins with different grafting densities and sulfonation degrees.The adsorption capacity of γ-globulin decreased with increasing alginate-grafting density (IC) from 160 to 230 mmol· L-1 at 0 mmol· L-1 NaCl because ofthe steric hindrance caused by the alginate-grafting layer.Effects of ionic strength (IS) indicated that the adsorption capacities of the resins with the IC value of 230-370 mmol·L-1 were much higher than CM Sepharose FF at 50-100 mmol· L-1 NaCl,and the uptake rate of Alg-FF-230 was about twice as much as that of CM Sepharose FF.This work demonstrated the important effects of alginate-grafting layer and IS in γ-globulin adsorption behavior,which would be helpful in the design of Alggrafted resins and the selection of proper IS condition for protein purification.