Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2

• 作者: Yuhua Wang Xuelin Zhou Jinyan Luo Suhui Lv Rui Liu Xuan Du Bei Jia Fengtong Yuan Heng Zhang Jiamu Du
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（6）
• 所属期刊栏目: Breakthrough Reports
• 页码范围: 1091-1096
• 页数: 6页
• 语种: 英文

摘要

RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HO-MOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which poten-tially links RdDM to H3K9me1 in maize.