First Isolation and Confirmation of Sterol Based on β-sitosterol Skeleton from the Leaves of Podocarpusnagi Planted in Fujian,Preliminary in vitro Anticancer Activity and the Crystal Structure

• 作者: YANG Yang YONG Jian-Ping OLAGOKE Zacchaeus Olatunde LU Can-Zhong
• 来源期刊: 结构化学
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: medicinal structural chemistry
• 页码范围: 653-658
• 页数: 6页
• 语种: 英文
• DOI: 10.14102/j.cnki.0254-5861.2011-2981

摘要

The crystal of(24R)-3β,5α-dihydroxy-24-ethyl-5α-cholestan-6-one(1)was isolated from the leaves of P.nagi planted in Fujian for the first time with the same skeleton as 26,27-dinorcholest-5-en-3-β-ol(2)andβ-sitosterol(3)that have been reported before.Compound 1 crystallizes in monoclinic,space group P21 with a =10.8482(4),b = 7.3671(3),c = 33.7860(15)?,β = 93.103(4)°,V = 2696.21(19)?3,Z = 75,Mr = 446.70,ρcalc =1.145 g/cm3,F(000)= 1032,μ = 0.572 mm-1,GOOF = 1.034,the final R = 0.0467 and wR = 0.1129 for 6989 observed reflections with I＞2σ(I).Compound 2 was selected to evaluate for their preliminary in vitro anticancer activity against four cancer cell lines for the first time.The results showed that compound 2 exhibited great inhibition against gastric cancer,breast cancer MCF-7,lung cancer A549 and Helacell lines with the inhibitions of 89.16％±1.17,97.02％±0.53,47.20％±2.58 and 36.89％±1.22 respectively at the concentration of 1.4×10-2 M,which means that we found the new anticancer compound in this plant medicine.