HYD-PEP06 suppresses hepatocellular carcinoma metastasis,epithelial-mesenchymal transition and cancer stem cell-like properties by inhibiting PI3K/AKT and WNT/β-catenin signaling activation

• 作者: Wei Tian Jiatong Li Zhuo Wang Tong Zhang Ying Han Yanyan Liu Wenfeng Chu Yu Liu Baofeng Yang
• 来源期刊: 药学学报（英文版）
• 年，卷（期）: 2021,（6）
• 所属期刊栏目: Original articles
• 页码范围: 1592-1606
• 页数: 15页
• 语种: 英文

摘要

HYD-PEP06,an endostatin-modified polypeptide,has been shown to produce effective anti-colorectal carcinoma effects through inhibiting epithelial--mesenchymal transition (EMT).However,whether HYD-PEP06 has similar suppressive effect on hepatocellular carcinoma (HCC) remained un-known.In this study,HYD-PEP06 inhibited metastasis and EMT but not proliferation in vitro.Cignal finder pathway reporter array and Western blot analysis revealed that HYD-PEP06 suppressed HCCLM3 cell metastasis and EMT by inhibiting the PI3K/AKT pathway.Moreover,HYD-PEP06 exerted anti-metastasis effects in HepG2 cancer stem-like cells (CSCs) via suppressing the WNT/β-catenin signaling pathway.Finally,in HCCLM3 tumor-bearing BALB/c nu/nu nude mice,HYD-PEP06 substantially suppressed tumor growth,lung metastasis and HCC progress.Our results suggest that HYD-PEP06 in-hibits the metastasis and EMT of HCC and CSCs as well,and thus has the potential as an agent for HCC treatment.