Fucoidans from Thelenota ananas with 182.4 kDa Exhibited Optimal Anti-Adipogenic Activities by Modulating the Wnt/β-Catenin Pathway

• 作者: LIU Yuanyuan WANG Na TIAN Yingying CHANG Yaoguang WANG Jingfeng
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Life and Fisheries Sciences
• 页码范围: 921-930
• 页数: 10页
• 语种: 英文

摘要

In this study,fucoidans were extracted from the sea cucumber Thelenota ananas (Ta-FUCs) by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC showing optimal anti-adipogenic activities was determined.Results of MTT and Oil red O staining analyses showed that the Ta-FUCs inhibited the prolif-eration and differentiation of 3T3-L1 adipocytes.Futhermore,Ta-FUCs significantly downregulated the key transcriptional factors,such as SREBP-1c,PPARy,and C/EBPα of adipocytes.The Ta-FUCs also activated Wnt/β-catenin pathway-related genes,such as β-catenin,LRP5,and FrZ.The Ta-FUCs suppressed lipid accumulation in 3T3-L1 adipocytes possibly by decreasing the expression of genes ACC,FAS,ME,GPAT,DGAT,and PILN,which are important in the synthesis of fatty acids and triglycerides;and by in-creasing the expression of genes PPARα,CPT-1α,and ACOX,which are crucial in fatty acid β-oxidation.The anti-adipogenic activi-ties initially increased and then declined with decreasing molecular weight.Among the Ta-FUCs,the 182.4kDa Ta-FUC exhibited optimal bioactivities.This study reports for the first time that Ta-FUCs can prevent obesity by regulating the differentiation and lipid accumulation of adipocytes.