Development of an efficient plant dual cytosine and adenine editor

• 作者: Rongfang Xu Fanna Kong Ruiying Qin Juan Li Xiaoshuang Liu Pengcheng Wei
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（9）
• 所属期刊栏目: Breakthrough Reports
• 页码范围: 1600-1605
• 页数: 6页
• 语种: 英文

摘要

An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6％, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7％ in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.