A Comparative Study About the Neuroprotective Effects of EPA-Enriched Phosphoethanolamine Plasmalogen and Phosphatidylethanolamine Against Oxidative Damage in Primary Hippocampal Neurons

• 作者: ZHU Yunfang ZHANG Tiantian DING Lin SHI Haohao XUE Changhu XIE Wancui CHE Hongxia WANG Yuming
• 来源期刊: 中国海洋大学学报（自然科学英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: Life and Fisheries Sciences
• 页码范围: 1207-1214
• 页数: 8页
• 语种: 英文

摘要

Oxidative stress is involved in the progression of neurodegenerative diseases.Previous evidences showed that plasma-logens could improve neurodegenerative diseases.In this study,we investigated the function of phosphoethanolamine plasmalogens enriched with EPA (EPA-pPE) and phosphatidylethanolamine enriched with EPA (EPA-PE) on oxidative damage prevention after hy-drogen peroxide (H2O2) and tert-butylhydroperoxide (t-BHP) challenge in primary hippocampal neurons.Results showed that neu-rons pretreated with EPA-pPE and EPA-PE demonstrated the ability to alleviate oxidative damage,which was proved by the in-creased cell viability.Moreover,the shape and number of neurons were more similar to those of the control group.Antioxidant acti-vity,apoptosis,as well as TrkB/ERK/CREB signaling pathway were investigated to explore the mechanisms.The results suggested that EPA-PE was superior to EPA-pPE in regulating mitochondrial apoptosis.EPA-pPE was more prominent than EPA-PE in upre-gulating TrkB/ERK/CREB signaling pathway.Phospholipids with EPA exerted neuroprotective effects via inhibiting oxidative stress,suppressing apoptosis,and regulating TrkB/ERK/CREB signaling pathway.Therefore,the results provide a scientific basis for utili-zation of phospholipids enriched with EPA on the treatment of neurodegenerative disease.