Influence of electroacupuncture with acupoints selected along meridians on gene expression of angiotensinogen of myocardial ischemia rats

• 作者: Zhe-Yan Sa Xiao-Xiang Zhu Long Wan Xiao-Hua Pan Cai-Lian Lan Jin-Sen Xu
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（7）
• 所属期刊栏目: Original article
• 页码范围: 6-10
• 页数: 5页
• 语种: 英文

摘要

Objective: To observe the effects of electroacupuncture along meridians on the expression of angiotensinogen (AGT) mRNA in myocardial tissue of myocardial ischemia (MI) rats. Methods: SD rats were randomly divided into four groups: control group, MI model group, Neiguan (PC6) point group (EA group) and non-acupoint (the lateral-superior side of the hip) group. Myocardial infarction was produced in rats with 85 mg/kg of isoproterenol administered subcutaneously twice at an interval of 24 h. Rats of EA group and non-acupoint group were treated with electroacupuncture, once daily for five consecutive days. Gene chip was used to detect RAS-related genes of myocardial tissue from the model group and the EA group. Cardiac index and pathological staining were observed of four groups. Real-time quantitative fluorescent PCR was used to detect the expression of AGT mRNA in cardiac tissue. Results: The expression of RAS-related genes were different between the model group and the EA group. The difference of AGT mRNA was the most significant. The cardiac index and AGT mRNA expression in the model group were significantly higher than those in the control group(P<0.01). While those in the EA group were significantly lower than model group(P<0.05). The cardiac index and the expressions of AGT mRNA in the non-acupoint group were significantly higher than those in the EA group(P<0.01), and there was no difference with the model group. Conclusion: Electroacupuncture along meridians can improve myocardial ischemia in rats, and its mechanism may be related to the down-regulation of AGT mRNA expression in myocardial tissue.