Effect of expansive duraplasty on spinal cord injury in rat model

• 作者: Yu-Bo Shi Ling Yu Wei-Chun Guo
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（7）
• 所属期刊栏目: Original article
• 页码范围: 16-20
• 页数: 5页
• 语种: 英文

摘要

Objective: To explore the effect of expansive duraplasty on spinal cord injury in rat model. Methods: The 42 adult SD rats were randomly divided into contusion injury group (control group), durotomy group and expansive duraplasty group, each group included 12 rats and the remaining 6 rats were used to create dural allograft. The function of the hind limbs of the animals was evaluated by BBB score. Hematoxylin-eosin (HE) staining was employed for lesion volume measurements, Luxol Fast Blue (LFB) staining were used for white matter sparing and Immunohistochemistry (IHC) analysis was performed to detect the glial fibrillary acidic protein (GFAP) expression. Results: The BBB scores of rats in expansive duraplasty group were higher in comparison with contusion injury group on day 7 and 21 after surgery, the differences were statistically significant (P<0.05), the BBB scores of rats in expansive duraplasty group were higher in comparison with durotomy group on day 14 and 28 after surgery, the differences were statistically significant (P<0.05); lesion volume measurements showed a decrease in expansive duraplasty group relative to contusion injury group, and the differences were statistically significant (P<0.05), analysis of Integrated Optical Density (IOD) values showed that white matter sparing was higher in expansive duraplasty group compared with contusion injury group and durotomy group, and the differences were statistically significant (P<0.01), the average number of GFAP positive (+) cells in expansive duraplasty group was reduced when compared with the other two groups, and the differences were statistically significant (P<0.05). Conclusion: Our findings suggest that expansive duraplasty following spinal cord injury may improve motor functions of the rats, reduce lesion volume, inhibit demyelination and reduce connective tissue scar formation.