Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes

• 作者: Jinjie Li Bo Tang Yingxiu Li Chenguang Li Minjie Guo Haiyang Chen Shichen Han Jin Li Qijin Lou Wenqiang Sun Peng Wang Haifeng Guo Wei Ye Zhanying Zhang Hongliang Zhang Sibin Yu Long Zhang Zichao Li
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（8）
• 所属期刊栏目: Molecular Physiology
• 页码范围: 1521-1536
• 页数: 16页
• 语种: 英文

摘要

Trichomes function in plant defenses against bi-otic and abiotic stresses;examination of glabrous lines, which lack trichomes, has revealed key as-pects of trichome development and function. Tests of allelism in 51 glabrous rice (Oryza sativa) accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice. Here, we report that OsSPL10 acts as a transcriptional regulator con-trolling trichome development. Haplotype and transient expression analyses revealed that var-iation in the approximately 700-bp OsSPL10 promoter region is the primary cause of the gla-brous phenotype in the indica cultivar WD-17993. Disruption of OsSPL10 by genome editing de-creased leaf trichome density and length in the NIL-HL6 background. Plants with genotype OsSPL10WD-17993/HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the glumes. HAIRY LEAF6 (HL6) encodes another transcription factor that regulates trichome ini-tiation and elongation, and OsSPL10 directly binds to the HL6 promoter to regulate its expression. Moreover, the transcript levels of auxin-related genes, such as OsYUCCA5 and OsPIN-FORMED1b, were altered in OsSPL10 overexpression and RNAi transgenic lines. Feeding tests using locusts (Locusta migratoria) demonstrated that non-glandular trichomes affect feeding by this herbivore. Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions.