Dietary rumen-protected L-arginine or N-carbamylglutamate attenuated fetal hepatic inflammation in undernourished ewes suffering from intrauterine growth restriction

• 作者: Hao Zhang Ying Zhang Yi Ma Mabrouk Elsabagh Hongrong Wang Mengzhi Wang
• 来源期刊: 动物营养（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: RUMINANT AND HERBIVORE NUTRITION
• 页码范围: 1095-1104
• 页数: 10页
• 语种: 英文

摘要

This study aimed to explore whether dietary rumen-protected L-arginine (RP-Arg) or N-carbamylglu-tamate (NCG) supplementation to feed-restricted pregnant ewes counteracts fetal hepatic inflammation and innate immune dysfunction associated with intrauterine growth retardation (IUGR) in ovine fetuses.On d 35 of pregnancy,twin-bearing Hu ewes (n =32) were randomly assigned to 4 treatment groups (8 ewes and 16 fetuses per group) and fed diets containing 100％ of the NRC requirements (CON),50％ of the NRC requirements (RES),RES + RP-Arg (20 g/d) (RESA),or RES + NCG (5 g/d) (RESN).At 08:00 on d 110 of gestation,fetal blood and liver tissue samples were collected.The levels of triglyceride,free fatty acid,cholesterol and β-hydroxybutyrate in the fetal blood of RESA and RESN groups were lower (P ＜ 0.05)than those of the RES group,but were higher (P ＜ 0.05) than those of the CON group.The interleukin (IL)-6 and IL-1 levels in fetal blood and liver tissue as well as the myeloid differentiation primary response 88(MyD88),transforming growth factor β (TGFβ),and nuclear factor kappa B (NF-κB) mRNA levels in the fetal liver were decreased (P ＜ 0.05) by the NCG or RP-Arg supplementation compared to the RES treatment.Similarly,the roll-like receptor (TLR)-4,MyD88,TGFβ,and p-c-Jun N-terminal kinase (JNK)protein levels in the fetal liver were reduced (P ＜ 0.05) in the NCG and RP-Arg-supplemented groups compared to the RES group.These results showed that dietary supplementation of RP-Arg or NCG to underfed pregnant ewes could protect against IUGR fetal hepatic inflammation via improving lipid metabolism,down-regulating the TLR-4 and the inflammatory JNK and NF-κB signaling pathways,and decreasing cytokine production in ovine fetal blood and liver tissue.