Arginine promotes myogenic differentiation and myotube formation through the elevation of cytoplasmic calcium concentration

• 作者: Lu Gong Xin Zhang Kai Qiu Linjuan He Yubo Wang Jingdong Yin
• 来源期刊: 动物营养（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: MOLECULAR NUTRITION
• 页码范围: 1115-1123
• 页数: 9页
• 语种: 英文

摘要

This study aimed to explore the mechanism underlying arginine-promoted myogenesis of myoblasts.C2C12 cells were cultured with a medium containing 0.1,0.4,0.8,or 1.2 mmol/L arginine,respectively.Cell proliferation,viability,differentiation indexes,cytoplasmic Ca2+ concentration,and relative mRNA expression levels of myogenic regulatory factors (MRF) and key Ca2+ channels were measured in the absence or presence of 2 chemical inhibitors,dantrolene (DAN,10 μmol/L) and nisoldipine (NIS,10 μmol/L),respectively.Results demonstrated that arginine promoted myogenic differentiation and myotube formation.Compared with the control (0.4 mmol/L arginine),1.2 mmol/L arginine upregulated the relative mRNA expression levels of myogenin (MyoG) and Myomaker at d 2 during myogenic induction(P ＜ 0.05).Cytoplasmic Ca2+ concentrations were significantly elevated by arginine supplementation at d 2 and 4 (P ＜ 0.05).Relative mRNA expression levels of Ca2+ channels including the type 1 ryanodine receptor (RyR1) and voltage-gated Ca2+ channel (Cav1.1) were upregulated by 1.2 mmol/L arginine during 2-d myogenic induction (P ＜ 0.01).However,arginine-promoted myogenic potential of myoblasts was remarkably compromised by DAN and NIS,respectively (P ＜ 0.05).These findings evidenced that the supplementation of arginine promoted myogenic differentiation and myotube formation through increasing cytoplasmic Ca2+ concentration from both extracellular and sarcoplasmic reticulum Ca2+.