Effect of electroacupuncture on the expression of CGRP and 5-HT in the serum and colon of IBS-C rats

• 作者: Xiao-Li Wu Fang Gao Sheng-Bing Wu Xing-Sheng Chen
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（10）
• 所属期刊栏目: Original article
• 页码范围: 25-29
• 页数: 5页
• 语种: 英文

摘要

Objective: To investigate the effects of electricity on the expression of procalcitonin (CGRP) and serotonin (5-HT) in serum and colon in rats with constipation-type irritable bowel syndrome (IBS-C). Methods: 50 rats were randomly divided into three groups: normal control group, model group, electroacupuncture group with four single points (tianshu electroacupuncture group, Colonic acupuncture group, Quchi electroacupuncture group, Shangjuxu acupoint group, and bilateral acupoint alternating between left and right), and non-meridian non-acupoint group (non-meridian non-acupoint group on both hip of electroacupuncture rats).The IBS-C model was replicated in the other three groups, except the normal control group, using the cold-fear-starvation synthesis method. After successful replication of the model, the four electroacupuncture single-point group and the non-acupoint group began electroacupuncture treatment once a day for 3 consecutive days. The visceral sensitivity and the expression of CGRP and 5-HT in serum and colon were observed. Results: Compared with the normal control group, the minimum volume threshold of abdominal retractile reflex (AWR) in the model group decreased (P<0.05), and the expressions of 5-HT and CGRP in serum and colon increased (P<0.05).Compared with the model group, AWR minimum volume threshold was increased in the electroacupuncture group with four single acupuncture points (P<0.05), the expression of 5-ht and CGRP in serum and colon was significantly decreased (P<0.05), and there was no significant change in each indicator in the non-acupuncture point group (P>0.05). Conclusion: Electroacupuncture combined with four single points can effectively improve the visceral sensitivity of IBS-C rats and regulate the levels of 5-HT and CGRP in serum and colon of rats, thus providing experimental basis for the clinical treatment of IBS-C.