m6A reader Igf2bp3 enables germ plasm assembly by m6A-dependent regulation of gene expression in zebrafish

• 作者: Fan Ren Ran Miao Rui Xiao Jie Mei
• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2021,（11）
• 所属期刊栏目: ARTICLES
• 页码范围: 1119-1128
• 页数: 10页
• 语种: 英文

摘要

Bucky ball (Buc) is involved in germ plasm (GP) assembly during early zebrafish development by regu-lating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m6A reader Igf2bp3 interacts and colocalizes with Buc in the GP.Similar to the loss of Buc,the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification,which can be partially restored by the injection of igf2bp3 mRNA.Igf2bp3 binds to m6A-modified GP-organizer and GP mRNAs in an m6A-dependent manner and prevents their degradation.These findings indicate that the functions of Igf2bp3,a direct effector protein of Buc,in GP mRNA expression and GP assembly involve m6A-dependent regulation;these results emphasize a critical role of m6A modification in the process of GP assembly.