Qu-2,a robust poplar suspension cell line for molecular biology

• 来源期刊: 林业研究（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: ORIGINAL PAPERS
• 页码范围: 733-740
• 页数: 8页
• 语种: 英文

摘要

Populus spp.have long been used as model woody plant species for molecular biology research.How-ever,tissues of poplar are often recalcitrant to experimental procedures for molecular studies.We generated a hormone autotrophic poplar suspension cell line from a hybrid of Pop-ulus alba×P.berolinensis'Yinzhong',named Qu-2.Qu-2 cells are suitable as a model biological system for study-ing woody plants.Qu-2 cells have many advantages over suspension cell lines derived so far from any other woody plants.Qu-2 cells are very easy to cultivate and can grow on several common plant culture media without the addi-tion of any plant hormone.They show exceptionally high growth rates,reaching an approximately 150-fold increase in biomass after one week of culturing.Another important unique characteristic of Qu-2 cells is that they can be cryo-preserved and readily reactivated.Qu-2 cells are suitable for molecular manipulations such as protoplast production,transient transformation,and RNA-seq analysis.Therefore,Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research,ranging from physiology to gene function.The Qu-2 cells will be made available to the plant community for research.