Efficient generation of homozygous substitutions in rice in one generation utilizing an rABE8e base editor

• 作者: Chuang Wei Chong Wang Meng Jia Hong-Xuan Guo Peng-Yu Luo Mu-Gui Wang Jian-Kang Zhu Hui Zhang
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（9）
• 所属期刊栏目: Breakthrough Reports
• 页码范围: 1595-1599
• 页数: 5页
• 语种: 英文

摘要

A new deaminase, TadA8e, was recently evolved in the laboratory. TadA8e catalyzes DNA deamination over 1,000 times faster than ABE7.10. We developed a high-efficiency adenine base editor, rABE8e (rice ABE8e), combining monomeric TadA8e, bis-bpNLS and codon optimization. rABE8e had substantially increased editing efficiencies at NG-protospacer adjacent motif (PAM) and NGG-PAM target sequences compared with ABEmax. For most targets, rABE8e exhibited nearly 100％ editing efficiency and high homozygous substitution rates in the specific editing window, especially at Positions A5 and A6. The ability to rapidly generate plant materials with homozygous base substitutions will benefit gene function research and precision molecular breeding.