Overexpression of Arabidopsis thaliana malonyl-CoA synthetase gene enhances cold stress tolerance by activating mitogen-activated protein kinases in plant cells

• 来源期刊: 林业研究（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: ORIGINAL PAPERS
• 页码范围: 741-753
• 页数: 13页
• 语种: 英文

摘要

Malonyl-CoA synthetases may modulate cell responses to abiotic stress by regulating stress-related sign-aling transduction pathways or activating expression of transcription factors.However,the molecular mechanism of cold stress tolerance enhanced by malonyl-CoA synthetase is not fully understood.Here,we report that overexpression of the Arabidopsis thaliana malonyl-CoA synthetase gene AAE13.1 resulted in increased cell viability and growth rate and decreased thiobarbituric acid reactive substances under cold stress in rice(Oryza sativa L.),tobacco(Nicotiana tab-acum),and slash pine(Pinus elliottii Engelm.).AAE13.1 was associated with cold stress tolerance by increasing the activity of ascorbate peroxidase,catalase,polyphenol oxi-dase,and peroxidase and the accumulation of acid phos-phatase and alkaline phosphatase.Among six rice mitogen-activated protein kinase(MAPK)genes examined,AAE13.1 overexpression increased the expression of OsMAPK genes during cold stress.AAE13.1 activated expression of stress-response genes OsMAPK1,OsMAPK2,and OsMAPK3,indi-cating that AAE13.1 enhances cold stress tolerance by regu-lating expression of MAPK genes in plant cells.These results increase our understanding of cold stress tolerance in species of monocotyledons,dicotyledons,and gymnosperms.