Temporal variation and sharing of antibiotic resistance genes between water and wild fish gut in a peri-urban river

• 作者: Zhen-Chao Zhou Ze-Jun Lin Xin-Yi Shuai Ji Zheng Ling-Xuan Meng Lin Zhu Yu-Jie Sun Wei-Chun Shang Hong Chen
• 来源期刊: 环境科学学报（英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: Regular articles
• 页码范围: 12-19
• 页数: 8页
• 语种: 英文

摘要

Antibiotic resistance genes (ARGs) as emergence contaminations have spread widely in the water environment.Wild fish may be recipients and communicators of ARGs in the water environment,however,the distribution and transmission of ARGs in the wild fish and relevant water environment were rarely reported.Here,we have profiled ARGs and bacterial communities in wild freshwater fish and relevant water in a peri-urban river using highthroughput qPCR and 16S rRNA gene sequence.A total of 80 and 220 unique ARG subtypes were identified in fish and water samples.Fish and water both showed significant ARG seasonal variations (P ＜ 0.05).The highest absolute abundance of ARGs in fish and water occurred in summer (1.32 × 109 copies per g,on average) and autumn (9.04 × 106 copies per mL),respectively.In addition,the bipartite network analysis showed that 9 ARGs and 1 mobile genetic element continuously shared in fish and water.Furthermore,bacteria shared in fish and water were found to significantly correlate with shard ARGs.The findings demonstrate that bacteria and ARGs in fish and water could interconnect and ARGs might transfer between fish and water using bacteria as a spreading medium.