The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s

• 作者: Dandi Li Mengxuan Wang Tongyao Mao Mingwen Wang Qing Zhang Hong Wang Lili Pang Xiaoman Sun Zhaojun Duan
• 来源期刊: 中国病毒学
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: RESEARCH ARTICLES
• 页码范围: 1187-1196
• 页数: 10页
• 语种: 英文

摘要

P[3]rotavirus (RV) has been identified in many species,including human,simian,dog,and bat.Several glycans,including sialic acid,histo-blood group antigens (HBGAs) are reported as RV attachment factors.The glycan binding specificity of different P[3]RV VP8*s were investigated in this study.Human HCR3A and dog P[3]RV VP8*s recognized glycans with terminal sialic acid and hemagglutinated the red blood cells,while bat P[3]VPS* showed neither binding to glycans nor hemagglutination.However,the bat P[3]VP8* mutant of C 189Y obtained the ability to hemagglutinate the red blood cells,while human P[3]HCR3A/M2-102 mutants of Y189C lost the ability.Sequence alignment and structural analysis indicated that residue 189 played an important role in the ligand recognition and may contribute to the cross-species transmission.Structural superimposition exhibited that bat P[3]VP8* model was quite different from the simian P[3]Rhesus rotavirus(RRV) P[3]VP8*,indicating that bat P[3]RV was relatively distinct and partially contributed to the no binding to tested glycans.These results promote our understanding of P[3]VP8*/glycans interactions and the potential transmission of bat/human P[3]RVs,offering more insight into the RV infection and prevalence.