Changes of NLRP1-ASC-Caspase-1 signaling pathways in peripheral blood monocytes from patients with atrial fibrillation

• 作者: Fei-Yu Zhu Qing-Mei Xu Jian Zhu Yang Tang Qin Gao Bi Tang Pin-Fang Kang Hong-Ju Wang
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（14）
• 所属期刊栏目: Original article
• 页码范围: 13-18
• 页数: 6页
• 语种: 英文

摘要

Objective: To detect the expression of (Peripheral blood mononuclear cells, PBMCs) NLRP3 signal pathway in peripheral blood monocytes of patients with chronic heart failure, and to explore the expression of NLRP3 signal pathway and its induced inflammatory response in PBMCs of patients with different types of chronic heart failure. Methods: patients with chronic heart failure (NYHA Ⅱ～Ⅳ), Ⅱ (nude 20), Ⅲ (nude 20) and Ⅳ (nude 20) admitted to our hospital from 2019 to 2020 were selected, and 20 normal subjects were selected as the control group. The peripheral venous blood of all subjects was collected, and the plasma and monocytes were extracted respectively. The monocytes were identified by magnetic beads sorting. The mRNA and protein expression levels of NLRP3, ASC and Caspase-1 in PBMCs were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blot, and the level of interleukin-1 β (IL-1 β) in plasma was detected by enzyme-linked immunosorbent assay ((ELISA)). Results: compared with the normal control group, the expression of NLRP3, ASC, Caspase-1 protein and mRNA in PBMCs of patients with grade Ⅱ, Ⅲ and Ⅳ increased. Compared with patients with grade Ⅱ, the expression of these indexes increased in patients with grade Ⅲ and Ⅳ. Compared with patients with grade Ⅲ, the expression of these indexes increased in patients with grade Ⅳ. Compared with the normal control group, the plasma levels of IL-1 β in patients with grade Ⅱ, Ⅲ and Ⅳ were higher than those in patients with grade Ⅱ, Ⅲ and Ⅳ (P < 0.05). The expression of these indexes in patients with grade Ⅲ and Ⅳ was higher than that in patients with grade Ⅲ (P < 0.05). Conclusion: the results suggest that NLRP3-ASC-Caspase-1 signal pathway may cause chronic inflammation in patients with heart failure and play a role in the progression of chronic heart failure.