Developmental Temporal Patterns and Molecular Network Features in the Transcriptome of Rat Spinal Cord

• 作者: Jian Yang Lili Zhao Sheng Yi Fei Ding Yumin Yang Yan Liu Yongjun Wang Mei Liu Chengbin Xue Lian Xu Leilei Gong Xinghui Wang Yu Zhang Bin Yu Guo-li Ming Xiaosong Gu
• 来源期刊: 工程（英文）
• 年，卷（期）: 2021,（11）
• 所属期刊栏目: Tissue Engineering-Article
• 页码范围: 1592-1602
• 页数: 11页
• 语种: 英文

摘要

The molecular network features of spinal cord development that are integral to tissue engineering remain poorly understood in placental mammals,especially in terms of their relationships with vital biological processes such as regeneration.Here,using a large-scale temporal transcriptomic analysis of rat spinal cord from the embryonic stage to adulthood,we show that fluctuating RNA expression levels reflect highly active transcriptional regulation,which may initiate spinal cord patterning.We also demonstrate that microRNAs(miRNAs)and transcriptional factors exhibit a mosaic profile based on their expression patterns,while differential alternative splicing events reveal that alternative splicing may be a driving force for the development of the node of Ranvier.Our study also supports the existence of a negative cor-relation between innate immunity and intrinsic growth capacity.Epigenetic modifications appear to per-form their respective regulatory functions at different stages of development,while guanine nucleotide-binding protein(G protein)-coupled receptors(including olfactory receptors(ORs))may perform pleio-tropic roles in axonal growth.This study provides a valuable resource for investigating spinal cord devel-opment and complements the increasing number of single-cell datasets.These findings also provide a genetic basis for the development of novel tissue engineering strategies.