Determination of pesticides in cannabis facilities is increasingly important as medicinal and recreational uses of cannabis products expand rapidly.We report a method involving wipe sampling,liquid chromatography separation,and tandem mass spectrometry,which enables determination of 82 pesticides out of the 96 regulated by Health Canada.To demon-strate an application of the method,we sampled and measured pesticides in two cannabis growing facilities,representing a non-certified and a certified site.We detected 41 pesti-cides in surface wipe samples at the non-certified site and 6 at the certified site.This study provides the first evidence showing pesticide occurrence on common surfaces in cannabis growing facilities and points to a need for routine monitoring and strict control of pesticide use in cannabis facilities.