The structural basis for glycerol permeation by human AQP7

• 作者: Li Zhang Deqiang Yao Ying Xia Fu Zhou Qing Zhang Qian Wang An Qin Jie Zhao Dianfan Li Yan Li Lu Zhou Yu Cao
• 来源期刊: 科学通报（英文版）
• 年，卷（期）: 2021,（15）
• 所属期刊栏目: Life Sciences
• 页码范围: 1550-1558
• 页数: 9页
• 语种: 英文

摘要

Human glycerol channel aquaporin 7(AQP7)conducts glycerol release from adipocyte and enters the cells in pancreatic islets,muscles,and kidney tubules,and thus regulates glycerol metabolism in those tissues.Compared with other human aquaglyceroporins,AQP7 shows a less conserved "NPA" motif in the center cavity and a pair of aromatic residues at Ar/R selectivity filter.To understand the structural basis for the glycerol conductance,we crystallized the human AQP7 and determined the structure at 3.7 ?.A substrate binding pocket was found near the Ar/R filter where a glycerol molecule is bound and stabilized by R229.Glycerol uptake assay on human AQP7 as well as AQP3 and AQP10 demonstrated strong glycerol transportation activities at the physiological condition.The human AQP7 structure,in combination with the molecular dynamics simulation thereon,reveals a fully closed conformation with its permeation pathway strictly confined by the Ar/R filter at the exoplasmic side and the gate at the cyto-plasmic side,and the binding of glycerol at the Ar/R filter plays a critical role in controlling the glycerol flux by driving the dislocation of the residues at narrowest parts of glycerol pathway in AQP7.