Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury

• 作者: Fei Yu Hai-Lin Xu Su-Ping Niu Ya-Jun Zhang Yu-Hui Kou Yu-Song Yuan
• 来源期刊: 中国神经再生研究（英文版）
• 学科: 医学
• 年，卷（期）: 2021,（8）
• 所属期刊栏目: Peripheral Nerve Injury and Neural Regeneration
• 页码范围: 1622-1627
• 页数: 6页
• 分类号: No.R446|R745|R363
• 语种: 英文

摘要

Peripheral nerves have a limited capacity for self-repair and those that are severely damaged or have significant defects are challenging to repair. Investigating the pathophysiology of peripheral nerve repair is important for the clinical treatment of peripheral nerve repair and regeneration. In this study, rat models of right sciatic nerve injury were established by a clamping method. Protein chip assay was performed to quantify the levels of neurotrophic, inflammation-related, chemotaxis-related and cell generation-related factors in the sciatic nerve within 7 days after injury. The results revealed that the expression levels of neurotrophic factors (ciliary neurotrophic factor) and inflammation-related factors (intercellular cell adhesion molecule-1, interferon γ, interleukin-1α, interleukin-2, interleukin-4, interleukin-6, monocyte chemoattractant protein-1, prolactin R, receptor of advanced glycation end products and tumor necrosis factor-α), chemotaxis-related factors (cytokine-induced neutrophil chemoattractant-1, L-selectin and platelet-derived growth factor-AA) and cell generation-related factors (granulocyte-macrophage colony-stimulating factor) followed different trajectories. These findings will help clarify the pathophysiology of sciatic nerve injury repair and develop clinical treatments of peripheral nerve injury. This study was approved by the Ethics Committee of Peking University People's Hospital of China (approval No. 2015-50) on December 9, 2015.