A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment

• 作者: Kun-Yang Wang Jie Shao Li-Gang Shao Jia-Jin Chen Gui-Shi Wang Kun Liu Xiao-Ming Gao
• 来源期刊: 中国物理B（英文版）
• 年，卷（期）: 2021,（5）
• 所属期刊栏目: ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS
• 页码范围: 426-432
• 页数: 7页
• 语种: 英文
• DOI: 10.1088/1674-1056/abda32

摘要

A new pressure-calibration method for calibrating the reduction of second harmonic (2f) amplitude caused by pressure broadening effect in sealed microbial growth environment is present.The new method combines with linewidth compen-sation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO2 in microbial growth.In order to verify the method,a simulation experiment is developed,in which the increasing CO2 concentration leads to the increasing pressure.Comparing with the relation between the traditional 2f amplitude and gas concentration,there is a monotonous relation between the calibrated 2f amplitude and CO2 concentration,particularly,a linear relation is present when the CO2 concentration is replaced with the CO2 particle number.In terms of microbial measurement,the growth of Escherichia coli is measured,and the culture bottle is sealed during the microbial growth process.The ex-perimental results show that,comparing to the microbial growth retrieved by traditional 2f amplitude,the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.