A new newt species,Tylototriton daloushanensis Zhou,Xiao & Luo,sp.nov.,is described from Guizhou,China,based on phylogenetic and morphological analyses.Phylogenetic analyses based on mitochondrial 16S and ND2 genes show that the new species is an independent lineage closely related to T.maolanensis,T.broadoridgus,T.dabienicus,T.anhuiensis,and T.wenxianensis.Morphologically,the new species is distinguished from its congeners by a combination of the following morphological characters:(1) large body size(SVL 64.7-83.6 mm in male and 70.5-100.3 mm in female);(2) head longer than wide,prominent bony ridges present;(3) snout rounded in dorsal view;(4) tail length less than snout-vent length in both males and females;(5) dark black body coloration,except for bright orange cranial region,palms and soles,vent region,and ventral ridge of tail;(6) relative length of toesⅢ>Ⅳ>Ⅱ>Ⅰ>Ⅴ;(7) fingertips reaching to between the eyes and nostrils when the forelimbs are stretched forward;(8) distal tip of the limbs greatly overlapping when the fore and hind limbs are pressed along the trunk;(9) presence of gular fold;(10) vertebral ridge slightly segmented,slightly flattened rib nodules,indistinct interspaces between rib nodules;and (11)lacking orange color markings on posterior parotoids and rib nodules.The found of the new species suggests that the species diversity of the genus Tylototriton may be underestimated.