Cancer biology deciphered by single-cell transcriptomic sequencing

• 作者: Yanmeng Li Jianshi Jin Fan Bai
• 来源期刊: 蛋白质与细胞
• 年，卷（期）: 2022,（3）
• 所属期刊栏目: REVIEW
• 页码范围: 167-179
• 页数: 13页
• 语种: 英文

摘要

Tumors are complex ecosystems in which heteroge-neous cancer cells interact with their microenvironment composed of diverse immune,endothelial,and stromal cells.Cancer biology had been studied using bulk genomic and gene expression profiling,which however mask the cellular diversity and average the variability among individual molecular programs.Recent advances in single-cell transcriptomic sequencing have enabled a detailed dissection of tumor ecosystems and promoted our understanding of tumorigenesis at single-cell reso-lution.In the present review,we discuss the main topics of recent cancer studies that have implemented single-cell RNA sequencing(scRNA-seq).To study cancer cells,scRNA-seq has provided novel insights into the cancer stem-cell model,treatment resistance,and can-cer metastasis.To study the tumor microenvironment,scRNA-seq has portrayed the diverse cell types and complex cellular states of both immune and non-im-mune cells interacting with cancer cells,with the pro-mise to discover novel targets for future immunotherapy.