Tracing PRX1+cells during molar formation and periodontal ligament reconstruction

• 作者: Xuyan Gong Han Zhang Xiaoqiao Xu Yunpeng Ding Xingbo Yang Zhiyang Cheng Dike Tao Congjiao Hu Yaozu Xiang Yao Sun
• 来源期刊: 国际口腔科学杂志（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: ORIGINAL ARTICLES
• 页码范围: 51-59
• 页数: 9页
• 语种: 英文

摘要

Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1+cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1+cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1+cells in PDL cells. The behavior of PRX1+cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1+cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1+cells;during transplanted teeth PDL reconstruction, PRX1+cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1+cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.