Effects of Wenglian Jiedu decoction on expression of serum IL-18, TNF-α and MUC2 in colon tissue of ulcerative colitis rats

• 作者: Yu-Ting Lu Gui-Ye An Xiao-Yan Zhang Qian Yang Yong-Li Huo Dian-Gui Li
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2022,（3）
• 所属期刊栏目: Original article
• 页码范围: 25-30
• 页数: 6页
• 语种: 英文

摘要

Objective: To study the effect of Wenglian jiedu decoction on the expression of serum interleukin-18 (IL-18), tumor necrosis factor-α (TNF--α) and colonic mucin-2 (MUC2) in rats with ulcerative colitis of turbid toxin type, and to explore its mechanism. Methods:60 Wistar male rats were randomly divided into model group, Wenglian jiedu decoction low, middle and high dose groups, mesalazine group and blank group. Except for the blank group, the rat model of ulcerative colitis was established by trinitrobenzenesulfonic acid (TNBS) / ethanol compound method. The general condition, body weight, fecal properties and occult blood of rats were observed 2 weeks after administration. The disease activity index (Diseaseactivityindex,DAI) was scored, the content of MUC2 protein was detected by immunohistochemical method, and the contents of IL-18 and TNF- α in serum were detected by enzyme-linked immunosorbent assay (Elisa). Results: compared with the blank group, the DAI score increased, the contents of IL-18 and TNF-α increased significantly, and the content of MUC2 protein decreased in the model group. Compared with the model group, the DAI score, the content of IL-18 and TNF- α decreased and the content of MUC2 protein increased in each treatment group. Compared with the mesalazine group, the DAI score and the contents of IL-18 and TNF-α in the high and middle dose groups had no significant difference. Compared with the low dose group, the DAI score in the mesalazine group and the high and middle dose group decreased, and the contents of IL-18 and TNF- α decreased. Conclusion: Wenglian jiedu decoction can regulate the immune system of intestinal epithelium by regulating the levels of serum IL-18 and TNF- α and increasing the expression of MUC2 protein, so as to achieve the purpose of repairing intestinal mucosa.