Genome-wide identification and transcriptional profiling analysis of PIN/PILS auxin transporter gene families in Panax ginseng

• 作者: Shuiming Xiao Yang Chu Yanjun Chen Qinghe Zhao Baosheng Liao Jingjing Zhang Yuan Gao Jiang Xu Shilin Chen
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Original Articles
• 页码范围: 48-57
• 页数: 10页
• 语种: 英文

摘要

Objective:Plant hormones act as chemical messengers in the regulation of plant development and meta-bolism.The production of ginsenosides in Panax hybrid is promoted by auxins that are transported and accumulated by PIN-FORMED(PIN)and PIN-LIKES(PILS)auxin transporters.However,genome-wide studies of PIN/PILS of ginseng are still scarce.In current study,identification and transcriptional profiling of PIN/PILS gene families,as well as their potential relationship with ginsenoside biosynthesis in Panax ginseng were investigated.Methods:PIN/PILS genes in P.ginseng was identified via in silico genome-wide analysis,followed by phy-logenetic relationships,gene structure,and protein profiles investigation.Moreover,previously reported RNA-sequence data from various tissues and roots after infection were utilized for PIN/PILS genes expres-sion pattern analysis.The Pearson's correlation analysis of specific PIN/PILS genes expression level and main ginsenoside contents were taken to reveal the potential relationship between auxin transports and ginsenoside biosynthesis in P.ginseng.Results:A genome-wide search of P.ginseng genome for homologous auxin transporter genes identified a total of 17 PIN and 11 PILS genes.Sequence alignment,putative motif organization,and sub-cellular local-ization indicated redundant and complementary biological functions of these PIN/PILS genes.Most PIN/PILS genes were differentially expressed in a tissue-specific manner,and showed significant correlations with ginsenoside content correspondingly.Eight auxin transporter genes,including both PIN and PILS subfamily members,were positively correlated with ginsenoside content(cor＞0.60;P-value＜0.05).The expression levels of eleven auxin transporter genes were increased dramatically in the early stage(0-0.5 DPI)after Cylindrocarpon destructans infection,accompanied with various overall expression pat-terns,implying the dynamic auxin transport in response to biotic stress.Conclusion:Based on the results,we speculate that the accumulation or depletion in temporal or spatial manner of auxin by PIN/PILS transporters involved in the regulation of HMGR activity and subsequent ginsenoside biosynthesis.