Confocal laser speckle autocorrelation imaging of dynamic flow in microvasculature

• 作者: E Du Shuhao Shen Anqi Qiu Nanguang Chen
• 来源期刊: 光电进展（英文版）
• 年，卷（期）: 2022,（2）
• 所属期刊栏目: Original Article
• 页码范围: 22-32
• 页数: 11页
• 语种: 英文
• DOI: 10.29026/oea.2022.210045

摘要

Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues. However, existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution. Here we re-port a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations. The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging, which is termed Line Scan Laser Speckle Autocorrelation Imaging (LS-LSAI). The technical advantages of LS-LSAI include high spatial resolution (～4.4 μm) for visualizing and quantifying blood flow in microvessels, as well as video-rate imaging speed for tracing dynamic flow.