Microbial inoculants and garbage fermentation liquid reduced root-knot nematode disease and As uptake in Panax quinquefolium cultivation by modulating rhizosphere microbiota community

• 作者: Pei Cao Xuemin Wei Gang Wang Xiaochen Chen Jianping Han Yuan Li
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Original Articles
• 页码范围: 58-69
• 页数: 12页
• 语种: 英文

摘要

Objective:To find a suitable ecological cultivation measure to solve the problem of root-knot nematode disease of Panax quinquefolium(Panacis Quinquefolii Radix)and the heavy metals accumulating in its roots.Methods:Three-year-old P.quinquefolium was treated with four different combinations of microbial inoc-ulant(MI)and garbage fermentation liquid(GFL)[the joint application of'TuXiu'MI and Fifty potassium MI(TF),the combination use of'No.1'MI and Fifty potassium MI(NF),'Gulefeng'poly-γ-glutamic acid MI(PGA),GFL],and the untreated control(CK).Here,high-throughput sequencing,ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition,heavy metals(As,Cd and Pb)content and ginsenoside content among different treatments.Results:The results revealed that different MIs and GFL could increase the root dry weight of P.quinque-folium,PGA enhanced it by 83.24％,followed by GFL(49.93％),meanwhile,PGA and GFL were able to les-sen root-knot nematode disease incidence by 57.25％and 64.35％.The treatment of PGA and GFL can also effectively reduce heavy metals in roots.The As content in GFL and PGA was decreased by 52.17％and 43.48％respectively,while the Cd and Pb contents of GFL and PGA was decreased somewhat.Additionally,the content of total ginsenosides was increased by 42.14％and 42.07％,in response to TF and NF,respectively.Our metagenomic analysis showed that the relative abundance of particular soil microbial community members related to the biocontrol of root-knot nematode disease and plant patho-gen(i.e.,Chaetomium in NF,Xylari in GFL,and Microascus in PGA),heavy metal bioremediation(Hyphomacrobium in PGA and Xylaria in GFL),and nitrogen fixation(Nordella and Nitrospira in TF)was sig-nificantly increased;notably,potential harmful microflora,such as Plectosaphaerella and Rhizobacter,were more abundant in the control group.Conclusion:MI and GFL could improve the quality of P.quinquefolium by modifying its rhizosphere micro-bial community structure and composition,both of them are beneficial to the development of ecological cultivation of P.quinquefolium.